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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: M2 macrophages promote myofibroblast differentiation of LR-MSCs and are associated with pulmonary fibrogenesis

Fig. 5

Blockade of the Wnt/β-catenin signaling pathway inhibits the development of bleomycin-induced pulmonary fibrosis. Mice (n = 10 in each group) were intraperitoneally injected with vehicle (10% DMSO/saline) or 5 mg/kg salinomycin three times a week as indicated 7 days after the administration of bleomycin. Mice were sacrificed at day 21 after bleomycin instillation. a Pulmonary fibrosis was determined by hematoxylin-eosin (H&E) staining and collagen I was revealed by Masson’s trichrome staining. Representative micrographs of histology are shown. b The expression of collagen I, α-smooth muscle actin (α-SMA), and Sca-1 in lung tissues was measured by western blotting. The expression levels were quantified with ImageJ (right panels; n = 3). GAPDH was used as a loading control. Results are normalized to the expression of each individual protein in the control which is given a value of 1 and are expressed as means ± SD (*p < 0.05 vs. Bleomycin + Vehicle)

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