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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: M2 macrophages promote myofibroblast differentiation of LR-MSCs and are associated with pulmonary fibrogenesis

Fig. 3

M2 macrophage infiltration predominates in fibrotic lungs of bleomycin-treated mice. Mice (n = 10 in each group) received either saline or bleomycin (5 mg/kg body weight) intratracheally. a Lung tissues obtained at the indicated time points postinjection were analyzed by q-PCR for mRNA expression of inducible nitric oxide synthase (iNOS) and arginase (Arg-1), marker genes for M1 and M2 polarization, respectively. b Representative gating strategy is shown to identify pulmonary macrophage subsets from whole lung-digests in bleomycin-treated mice. M1 and M2 macrophages were analysed by using flow cytometry for the M2 macrophage marker, CD206 in the F4/80+ cell fraction (n = 3). c and d Purified primary LR-MSCs were cocultured with M1 macrophages or M2 macrophages polarized from mouse macrophages (RAW264.7) in vitro, or with control medium, in a transwell system. The expression of α-smooth muscle actin (α-SMA) and collagen I in LR-MSCs was measured by immunofluorescence assay c and western blotting (d)

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