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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Regulation of MRTF-A by JMY via a nucleation-independent mechanism

Fig. 4

Truncated JMY competes with MRTF-A for actin binding. a: NIH 3 T3 cells co-expressing Flag-actin-WT and myc-JMY constructs were serum-starved for 24 h post transfection. a: Purified MRTF-A (2–261) was added to the lysates prior to immunoprecipitation with anti-Flag magnetic beads. Binding of proteins (IP panel) as well as the input control (Input panel) were detected with the indicated antibodies. Both lanes for MRTF-A (2–261) or Flag-actin-WT are from the same immunoblot, respectively. b: Quantification of precipitated MRTF-A (2–261) by Flag-actin upon co-expression of various JMY constructs. c: Immunoprecipitation with anti-Flag magnetic beads. Binding of proteins (IP panel) as well as the input control (Input panel) were detected with the indicated antibodies. Both lanes for myc-JMY constructs or Flag-actin-WT are from the same immunoblot, respectively. d: Quantification of precipitated JMY variants by Flag-actin. All data were normalized to myc-JMY-f.l. Error bars, s.e.m., n = 3

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