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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: SOX2 as a novel contributor of oxidative metabolism in melanoma cells

Fig. 4

SOX2 regulates HIF1α expression in SSM2c, A375-M6 and 501-Mel melanoma cells. a) (Left) Representative image of HIF1A promoter showing the sequence and the position of the putative SOX2-binding sites (BS) relative to the transcriptional start site (TSS). (Right) Chromatin Immunoprecipitation (ChIP) assay showing SOX2 binding at HIF1A promoter; Actin promoter was used as negative control and set to 1. p < 0.01, Two-way ANOVA. b) Quantification of dual-luciferase reporter assay in SSM2c cells. Relative luciferase activities were Firefly/Renilla ratios, with the level induced by control equated to 1. Data represent mean ± s.e.m. p < 0.001 vs control, One-way ANOVA. N = 4. c, d) qPCR (c) and Western blot (d) of HIF1α in SSM2c LV-shSOX2 compared to LV-c. Quantification of SOX2 and HIF1α protein is shown in italic. p < 0.01, T-test. N = 4. e, f) qPCR (e) and Western blot (f) of HIF1α in A375-M6 LV-shSOX2 compared to LV-c. Quantification of SOX2 and HIF1α protein is shown in italic. p < 0.05, T-test. N = 4. g, h) qPCR (g) and Western blot (h) of HIF1α in 501-Mel pBABE-SOX2 compared to pBABE-c. Quantification of SOX2 and HIF1α protein is shown in italic. p < 0.01, T-test. N = 4. HSP90 was used as loading control

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