Fig. 3
From: SOX2 as a novel contributor of oxidative metabolism in melanoma cells
Modulation of SOX2 expression induces metabolic changes in SSM2c and in 501-Mel melanoma cells. a) Western blot analysis of SOX2 expression in SK-MEL-2, SK-MEL-5, SK-MEL-28, A375-M6, 501-Mel and SSM2c melanoma cells. β-actin was used as loading control. b, c) qPCR (b) and Western blot (c) of SOX2 in SSM2c silenced for SOX2 (LV-shSOX2) compared to control (LV-c). Quantification of SOX2 protein is shown in italic. p < 0.05, T-test. N = 3. d) Quantification chart of lactate production of SSM2c LV-shSOX2 compared to LV-c. p < 0.05, T-test, N = 3. e) qPCR of a panel of glycolysis- and OxPhos-related genes in SSM2c LV-shSOX2 compared to LV-c. *p < 0.05, **p < 0.01, ***p < 0.001, T-test. N = 3. f, g) qPCR (f) and Western blot (g) of SOX2 in 501-Mel with SOX2 overexpression (pBABE-SOX2) compared to control (pBABE-c). Quantification of SOX2 protein is shown in italic. p < 0.01, T-test. N = 3. h) Quantification chart of lactate production of 501-Mel pBABE-SOX2 compared to pBABE-c. p < 0.01, T-test, N = 3. i) qPCR of a panel of glycolysis- and OxPhos-related genes in 501-Mel pBABE-SOX2 compared to pBABE-c. *p < 0.05, **p < 0.01, ***p < 0.001, T-test. N = 3