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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Akt1 inhibition promotes breast cancer metastasis through EGFR-mediated β-catenin nuclear accumulation

Fig. 5

Knockdown of Akt1 induced the activation of ERK signaling in breast cancer cells, thus resulting in β-catenin nuclear accumulation and cancer cell invasion. (a) Western blot analysis showed that knockdown of Akt1 enhanced the activation of ERK signaling pathway in MCF-7 and MDA-MB-231 cells, n = 5 per group. (b) Western blot analysis showed that Akt1 siRNA-induced β-catenin nuclear accumulation was almost completely blocked by ERK inhibitor U0126 (20 μM) after 24 h co-incubation in MCF-7 and MDA-MB-231 cells, n = 5 per group. (c-e) Transwell assay with Matrigel demonstrated that ERK inhibitor U0126 (20 μM for 24 h) reversed Akt1 siRNA-induced breast cancer invasion in MCF-7 (c, d) and MDA-MB-231 cells (c, e). ***p < 0.001, one-way ANOVA, n = 5 per group

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