Fig. 3
From: Four-octyl itaconate activates Keap1-Nrf2 signaling to protect neuronal cells from hydrogen peroxide
OI inhibits H2O2-induced oxidative stress in neuronal cells. SH-SY5Y cells (a-d) or the primary murine neurons (e-f) were pretreated for 30 min with 4-octyl itaconate (OI, 25 μM), followed by stimulation of H2O2 (300 μM) for indicated time, relative DCF fluorescent intensity (a and e) and relative superoxide level (b and f) were tested; The lipid peroxidation (c) and DNA damage (d) were tested by TBAR activity assay and p-H2AX FACS assay, respectively. Bars stand for mean ± standard deviation (S.D., n = 5). “Vehicle” stands for PBS control (Same for all Figures). * P < 0.05 vs. “Ctrl” cells. # P < 0.05 vs. H2O2 treatment only