Fig. 2
From: Four-octyl itaconate activates Keap1-Nrf2 signaling to protect neuronal cells from hydrogen peroxide
Four-octyl itaconate attenuates H2O2-induced neuronal cell death and apoptosis. SH-SY5Y cells (a-h) or the primary murine neurons (i-k) were pretreated for 30 min with applied concentration of 4-octyl itaconate (OI), followed by stimulation of H2O2 (300 μM) for indicated time, cell viability, cell death and apoptosis were tested by the listed assays mentioned in the text (a-k). Expression of listed proteins were quantified and normalized to the loading control (d and k). Bars stand for mean ± standard deviation (S.D., n = 5). * P < 0.05 vs. “Ctrl” cells. # P < 0.05 vs. H2O2 treatment only