Fig. 5
From: USP17 is required for trafficking and oncogenic signaling of mutant EGFR in NSCLC cells
(a/c) HCC827 and H1975 cells were transfected as indicated. 48 h post transfection, whole cell lysates were harvested and levels of cleaved PARP, cleaved caspase 9, cleaved caspase 3, and BCL-2 were assessed by immuno-blotting using anti-cleaved PARP, anti-caspase 9, anti-cleaved caspase 3, anti-BCL-2, in addition to α-tubulin, as a loading control. (b/d) HCC827 and H1975 cells were transfected as indicated. Caspase-3/7 activity levels were assessed 48 h post-transfection. ** p < 0.01, *** p < 0.001