Fig. 3

Modified preservative fluid preserved autologous blood promotes wound healing in diabetic mice through up-regulating H19 expression. a, the modified preservative fluid preserved autologous blood could significantly increase the speed of wound healing in diabetic mice; N = 8; b HE staining (× 400) was used to observe histopathological changes during wound healing at different time points in response to standard and modified preservative fluid preserved autologous blood; scale bar = 25 μm; c immunohistochemical staining (× 400) showing that modified preservative fluid preserved autologous blood in diabetic mice could significantly increase the number of fibroblasts; scale bar = 25 μm; N = 8; d western blot analysis was used to detect the expression of wound healing related proteins, showing that modified preservative fluid preserved autologous blood significantly elevated the expression of VEGF, SDF, SCF, MMP-1, MMP-2 and MMP-3; e speed of wound healing was inhibited after injection of sh-H19 lentivirus; N = 8; f HE staining (× 400) was used to observe histopathological changes during wound healing at different time points in response to injection of sh-H19 lentivirus; scale bar = 25 μm; g immunohistochemical staining (× 400) showing that number of fibroblasts was decreased after injection of sh-H19 lentivirus; scale bar = 25 μm; N = 8; h western blot analysis showing that the expression of VEGF, SDF, SCF, MMP-1, MMP-2 and MMP-3 was reduced in response to injection of sh-H19 lentivirus; *, p < 0.05; The above results were measurement data, which were presented as the mean ± standard deviation. Comparisons between two groups were analyzed by t-test. The speed of wound healing was analyzed by two-way analysis of variance. The experiment was repeated 3 times; standard, standard preservative fluid preserved autologous blood; modified, modified preservative fluid preserved autologous blood; DM, diabetes mellitus; NC, negative control; VEGF, vascular endothelial growth factor; SDF, stromal cell-derived factor; SCF, stem cell factor; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HE, hematoxylin-eosin