Fig. 2

Modified preservative fluid preserved autologous blood enhanced the activation of fibroblasts in diabetic mice through up-regulating H19 expression. a western blot analysis showing that modified preservative fluid preserved autologous blood increased the expression of fibroblast activation marker proteins (FAP, Col1A1, α-actin and α-SMA); b Masson staining (× 400) showing that modified preservative fluid preserved autologous blood increased collagen content of skin tissues; scale bar = 25 μm; N = 8; c RT-qPCR showing that expression of H19 in fibroblasts was significantly increased/decreased after transfection of H19 overexpression/siRNA H19 plasmids; d western blot analysis showing that expression of fibroblast activation related proteins (FAP, Col1A1 and α-SMA) was significantly increased/decreased in response to H19 overexpression/siRNA H19 transfection; e EDU staining (× 400) showing that the proliferation ability of fibroblasts was significantly increased/decreased in response to H19 overexpression/siRNA H19 transfection; scale bar = 25 μm; f Scratch test showing that the migration ability of fibroblasts was significantly increased/decreased in response to H19 overexpression/siRNA H19 transfection; *, p < 0.05; The results were measurement data, which were presented as the mean ± standard deviation. Comparisons between two groups were analyzed by t-test. The experiment was repeated 3 times; standard, standard preservative fluid preserved autologous blood; modified, modified preservative fluid preserved autologous blood; RT-qPCR, reverse transcription quantitative polymerase chain reaction; DM, diabetes mellitus; NC, negative control; α-SMA, α-smooth muscle actin; EDU, 5-ethynyl-2′-deoxyuridine