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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Conditional ablation of p130Cas/BCAR1 adaptor protein impairs epidermal homeostasis by altering cell adhesion and differentiation

Fig. 5

p130Cas impairs cell-cell adhesion dynamics. (a) Phospho-tyrosine immunoprecipitates from confluent untreated and calcium-treated WT and p130CasKO keratinocytes blotted with E-cadherin and beta-catenin antibodies. IgG indicates the immunoprecipitation control. Cell extracts are shown at the bottom (left panels). Densitometric analysis of protein levels of at least three independent experiments is shown in the right panels (p* < 0.05, **p < 0.01). (b) E-cadherin immunoprecipitates from the same extracts as in (a) blotted with alpha-catenin antibodies (left panels). Densitometric analysis of protein levels of at least three independent experiments is shown on the right panel (p* < 0.05). (c) Triplicate samples of primary keratinocytes from WT of p130Cas KO mice were kept under low calcium condition or treated with calcium for 1, 3 and 8 h. Data are expressed as percentage of single cells released by mechanical disruption after dispase treatment versus total number of cells recovered after subsequent treatment of the same sample with trypsin. (d) Representative images of actin (green) and vinculin (red) staining of WT and p130CasKO keratinocytes in LCM or 1 h after calcium treatment (63X). Experiments in c and d were performed three times. (e) Representative images of wound healing experiments at time 0 and after 48 h (10X) (left panel), and relative quantification (right panel) by using nonparametric Mann-Whitney test (***p < 0.001). Data are expressed as median ± range of three independent experiments

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