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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Conditional ablation of p130Cas/BCAR1 adaptor protein impairs epidermal homeostasis by altering cell adhesion and differentiation

Fig. 4

p130Cas is required for proper ECM and cell-cell adhesion. (a) Adhesion quantification of freshly isolated WT and p130CasKO primary keratinocytes on laminin, fibronectin and collagen after 12 h of cell adhesion. Data are expressed as mean ± S.D. of five independent experiments (**p < 0.01). (b) Western blotting analysis for phosho-Src (pSrc), c-Src, phospho-ERK1/2 MAPKs (pERK1/2) and ERK1/2 MAPKs from confluent untreated and calcium-treated WT and p130CasKO keratinocytes. (c) Densitometric analysis of protein levels of at least three independent experiments is shown (p* < 0.05, **p < 0.01). Activation of Src and ERK1/2 MAPKs was normalized on c-Src and ERK1/2 levels, respectively, as loading control. (d, e) Representative images of E-cadherin (d) and beta-catenin (e) stainings in untreated and calcium-treated WT and p130CasKO keratinocytes. DAPI stained nuclei (63X magnification) (left panels). Quantification of immunofluorescent staining of membrane-associated E-cadherin and beta-catenin is shown in the right panels. The data represented the mean ± S.D. of 4 independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001) (field equal to 50 μm)

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