Fig. 3

p130Cas loss affects keratinocytes differention. (a) Representative images of confluent WT and p130CasKO keratinocytes in low calcium condition and after 8 and 24 h of calcium treatment (20X magnification). (b) Western blot analysis of early and late differentiation markers of WT and p130CasKO keratinocytes either untreated or calcium treated. (c) Densitometric analysis of protein levels of at least three independent experiments is shown (p* < 0.05, **p < 0.01). (d) Representative images and quantification of filaggrin fluorescence staining of WT and p130CasKO 3-day-old pups skin (left and right panel, respectively) (40X). (e) Representative images and quantification of loricrin fluorescence staining of WT and p130CasKO 3-day-old pups skin (left and right panel, respectively) (40X). Data are expressed as mean ± S.D. of three independent experiments