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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Conditional ablation of p130Cas/BCAR1 adaptor protein impairs epidermal homeostasis by altering cell adhesion and differentiation

Fig. 2

p130Cas deletion alters the balance between epidermal proliferation and differentiation. (a, b) Representative images of Ki67 staining from WT and p130CasKO of 3-day-old pups skin (20X). Quantification was performed by counting Ki67 basal positive cells on the entire images (field equal to 350 μm). The data represented the mean ± S.D. from 10 WT and 10 p130CasKO mice (*p < 0.05). Inset were taken at 100X magnification. (c, d) Representative images of PCNA staining from WT and p130CasKO of 3-day-old pups skin (20X). Quantification was performed by counting PCNA basal positive cells on the entire images (field equal to 350 μm). The data represented the mean ± S.D. from 10 WT and 10 p130CasKO mice (*p < 0.05). Inset were taken at 100X magnification. (e) WT and p130CasKO keratinocytes were plated in triplicate on collagen coated dishes and counted every day for 72 h. Data are expressed as mean ± S.D. of results from three independent experiments (**p < 0.01). (f) Representative images of first and second progeny of WT and p130CasKO keratinocytes cultured under clonogenic conditions for 12 days, followed by rhodamine-B staining. (g) SEM of colonies of the indicated sizes. 100 colonies were counted from duplicate plates and classified based on morphological parameters. Data represent two independent experiments. (h) Untreated and calcium-treated WT and p130CasKO keratinocytes extracts blotted for ΔNp63 (Tubulin as loading control), phospho-YAP, YAP, Cyclin D1 (GAPDH as the loading control). (i) Densitometric analysis of protein levels of at least three independent experiments is shown (*p < 0.05, p** < 0.01, ***p < 0.001)

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