Fig. 1

Conditional deletion of p130Cas in the mouse epidermis. (a) Schematic representation of p130Cas/BCAR1 gene targeting strategy. (b) Epidermis isolated from WT (mice 2, 6) and p130CasKO (mice 1, 3, 4 and 5) 3 days old pups were lysed and cell extracts were subjected to western blotting protein analysis. Representative images of WT and p130CasKO mice at birth (PO) (right panels). (c) Representative haematoxylin/eosin stained sections from WT and p130CasKO skins of 3 days old pups (10X) (left panels). Higher magnification insets of representative haematoxylin/eosin stained section from WT and p130CasKO skins (20X) (right panels). (d) Quantification of epidermis thickness in WT and p130CasKO. The data represented the mean ± S.D. from 10 WT and 10 p130CasKO mice (**p < 0.01). (e) Representative images of K14 and K1 fluorescence staining of WT and p130CasKO 3-day-old pups skin (40X) (left panel). Quantification of immunofluorescent stainings is shown in the right panels. K1 staining intensity was normalized for K14 staining intensity. The data represented the mean ± S.D. from 6 WT and 6 p130CasKO mice (**p < 0.01) (field equal to 200 μm)