Fig. 8

Akt directly interacted with Smad3. a: The left two shows p-Smad3 protein from cell lysates with or without insulin (100 nM, 15 min) was pulled down using Smad3 specific antibody. The right shows its reciprocal protein p-Akt was detected by p-Akt specific antibody. b. The left two shows p-Akt protein from cell lysates with or without insulin (100 nM, 15 min) was pulled down by p-Akt specific antibody. The right shows the p-Smad3 was detected by p-Smad3 specific antibody. p-Smad3: 56 kDa, p-Akt: 60 kDa