Fig. 3
From: SRC inhibition prevents P-cadherin mediated signaling and function in basal-like breast cancer cells
Dasatinib treatment promotes the recovery of E-cadherin function with stabilization of the E-cad/p120ctn complex to the cell membrane. a Dual Immunofluorescence for anti-p120ctn antibody (green), F-actin (red), E-cadherin (green) and DAPI (blue) in MCF-7/AZ.Pcad cells after 100 nM dasatinib treatment for 48 h. Scale bar = 50 μm. b Internuclear profile for p120ctn and E-cadherin, obtained through the computational analysis of confocal immunofluorescent images. P-values indicate significant differences observed in the membrane mean fluorescent intensity for p120ctn between MCF-7/AZ.Pcad treated with DMSO versus 100 nM dasatinib, 48 h. c Quantification of the active Rac1-GTP, measured by a G-Lisa assay, was performed to measure Rac1 activity in P-cadherin overexpressing cells (MCF-7/AZ.Pcad and BT20) treated with DMSO and dasatinib. d Proximity ligation assay for E-cadherin and p120ctn, for MCF-7/AZ.Pcad treated with DMSO versus 100 nM dasatinib during 48 h. e 72 h slow aggregation assay images for dasatinib treated MCF-7/AZ.Pcad cells with inhibition of E-cadherin function by MB2 antibody. The images shown are representative ones. P-values are indicated in the figure and considered statistically significant when < 0.05