Fig. 1
From: SRC inhibition prevents P-cadherin mediated signaling and function in basal-like breast cancer cells
P-cadherin overexpression associates with SRC activation correlating with an aggressive behavior. a Gene expression profile of SRC family members from BCC lines retrieved from the CCLE database [20]. Unsupervised hierarchical clustering based on classical cadherins expression (CDH1, CDH2, CDH3), subdivide the BCC lines into their molecular subtype (Luminal, Basal A and Basal B). b Box-plot representation of CDH1, CDH2 and CDH3 gene expression values in the different breast cancer molecular subtypes. c Box-plot for SRC associated genes expression in the Luminal and Basal A BCC subtypes. P-values indicate significantly differences observed in gene expression profile from Luminal and Basal A BCC lines. d Western blot analysis of P-cadherin, E-cadherin, p120ctn, pSRC(Tyr416), total SRC and β-actin proteins after P-cadherin overexpression and P-cadherin silencing, respectively. e Dual immunofluorescence confocal images using an anti-p120ctn antibody (green), F-actin (red) and DAPI (blue). f Internuclear profile for p120ctn and E-cadherin, obtained through the computational analysis of immunofluorescence images from MCF-7/AZ BCC model. P-value indicate significant differences observed in the membrane mean fluorescent intensity for p120ctn between MCF-7/AZ.Mock versus MCF-7/AZ.Pcad cells. g Quantification of the active Rac1-GTP, measured by a G-Lisa assay after P-cadherin overexpression and P-cadherin silencing, respectively. h Average values of Work (J), representing the cell-cell adhesion strength, using AFM Force Spectroscopy analysis. Student’s t-test was used to determine statistically significant differences, and P-values are indicated in the figure. i Representative Images of time-lapse microscopy for BCC spheroids embedded in collagen type I. Scale bar = 50 μm. j Box-plot quantification of the extension (μm) of invasive protrusions. k Box-plot quantification of the number of invasive protrusions. l Representation of the percentage of cases positive for both pSRC(Tyr416) and P-cadherin expression. P-value was calculated by Chi-square test in order to estimate the relationship between staining patterns of P-cadherin and pSRC(Tyr416)