Table 3 qPCR primers
Gene-name (Accession number) | Forward primer (5‘→3‘) | Reverse primer (5‘→3‘) | Amplicon size (bp) | Efficiency (%) |
|---|---|---|---|---|
Actb (NM_007393.5) | CGTGCGTGACATCAAAGAGAA | GGCCATCTC CTGCTCGAA | 61 | 102 |
Ctnnb1 (β-catenin) (NM_001165902.1) | TTAAACTCCTGCACCCACCAT | AGGGCAAGGTTTCGAATCAA | 73 | 103 |
B2m (NM_009735.3) | AAATGCTGAAGAACGGGAAAA | ATAGAAAGACCAGTCCTTGCTGAAG | 77 | 96 |
Myc (NM_001177352.1) | CGTTGGAAACCCCGCAGA | TACGGAGTCGTAGTCGAGGT | 86 | 97 |
Ccnd1 (NM_007631.2) | ACTGCCGAGAAGTTGTGCAT | AAGCAGTTCCATTTGCAGCAG | 72 | 101 |
Gapdh (NM_008084.3) | CGGCCGCATCTTCTTGTG | CCGACCTTCACCATTTTGTCTAC | 59 | 100 |
Lcn2 (NM_008491.1) | CCACCACGGACTACAACCAG | AGCTCCTTGGTTCTTCCATACA | 100 | 98 |
Lcn2-R (Slc22a17) (NM_021551.4) | CAGCCACCTCCTAACCGCTGTG | CTCCCACTAGGATCAAAGGCTGCT | 86 | 110 |
Wnt4 (NM_009523.2) | GAGAAGTGTGGCTGTGACCGG | ATGTTGTCCGAGCATCCTGACC | 77 | 92 |
Wnt5a (NM_009524.3) | CTCCTTCGCCCAGGTTGTTATAG | TGTCTTCGCACCTTCTCCAATG | 196 | 117 |