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Table 1 Primary antibodies

From: Tonicity inversely modulates lipocalin-2 (Lcn2/24p3/NGAL) receptor (SLC22A17) and Lcn2 expression via Wnt/β-catenin signaling in renal inner medullary collecting duct cells: implications for cell fate and bacterial infection

Immunogen Host species Manufacturer Catalog # Application Dilution
Lcn2 (rat) goat RD Systems AF3508 IB 1:200-300
Lcn2 (mouse) rabbit Abcam Ab63929 IF 1:250-500
Lcn2-R (C-term) (rat) rabbit Immunoglobe Ig-1086 IB 1:250-2,000
Lcn2-R (N-term) (rat) rabbit Immunoglobe Ig-1095 IF 1:100-1000
β-actin mouse Sigma-Aldrich A5316 IB 1:20,000
β-catenin (mouse) mouse BD Biosciences 610153 IB 1:2,000-5,000
GSK-3β (rat) mouse BD Biosciences 610201 IB 1:500
Phospho-GSK-3 β (Ser9) (human) rabbit Cell Signaling Technology 9336 IB 1:500
Na+,K+-ATPase α1-subunit (human) rabbit Cell Signaling Technology 3010S IB IF 1:250-500 1:100
FLAG-M2 mouse Sigma-Aldrich F3165 IB 10μg/ml
  1. IB = immunoblotting. IF = immunofluorescence