Fig. 7
MIF contributes to resistance to apoptosis in BLEL primary cells. (a) Immunoblotting showing the influence of MIF on Bax and Bcl-2 proteins expression. (b) Representative images of immunostaining showing the influence of MIF on Bcl-2 expression in BLEL primary cells. (c) Flow Cytometry graphs showing viable cells (lower-left quadrant), early apoptotic cells (lower- right quadrant), late apoptotic cells (upper-right quadrant) and nuclear debris (upper-left quadrant). (d) MIF prevents BLEL primary cells from DMSO-induced apoptosis. In (a & c) BLELp1 and BLELp2 cells were seeded at a density of 106 cells/dish, and at a density of 2 × 104 cells/well in 24 well plate in (b & d). Cells were treated with MIF (200 ng/ml), ISO-1 (100 μM) or without MIF nor ISO-1 for 72 h or 1 week in (a), and for 1 week in (b & c). In (d), cells were pre-treated with or without MIF (200 ng/ml) for 48 h before exposition to 1% DMSO (78.13 g/mol) for 24 h together with or without MIF (200 ng/ml). Data were plotted as Mean ± SEM. * P-value < 0.05; ** P-value < 0.01; *** P-value < 0.001; no stars for P-value > 0.05. Original magnification: (b) 20× and (d) 4×