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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells

Fig. 4

a PCR analysis was performed with mcherry, eGFP, MSC stem-like markers CD73, CD90, CD105, and fusion-associated factors syncytin-2 and MFSD-2A expression in the parental SK-OV-3cherry and MSCGFP populations as compared to the two hybrid populations SK-hyb1 and SK-hyb2, respectively. Unaltered GAPDH transcripts served as loading control. b Proliferative capacity of the parental SK-OV-3cherry cells was compared to the SK-hyb1 and SK-hyb2 hybrid populations by cell counting of initially seeded 104 cells/well in 24-well plates for 24 h up to 72 h, respectively. Data represent the mean + s.d. (n = 3). c Cell cycle analysis was performed in steady state SK-hyb1 cells and compared to the parental control populations SK-OV-3cherry and MSCGFP using the FlowJo V10 software. Cell cycle sub-populations of similar relative fluorescence intensities were matched by the dotted line indicating that the DNA content of MSC in G2/M corresponded to SK-hyb cells in G0/G1 phase. d Tumor weight of SK-OV-3GFP-induced tumors (n = 4) was measured following dissection of the solid subcutaneous primary tumors. No tumors were detectable after transplantation of either SK-hyb1 or SK-hyb2 cell populations

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