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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: A role for RASSF1A in tunneling nanotube formation between cells through GEFH1/Rab11 pathway control

Fig. 5

Exosomes released by RASSF1A depleted cells affect TNT formation. a Representative images and b–c quantification of cytoplasmic vesicles number and diameter (μm) by electron microscopy in HBEC-3 cells after transfection with control or RASSF1A RNAi. The vesicles are show with yellow arrowheads. d Quantification of the exosome release in control and RASSF1A knockdown HBEC-3 cells. e Representative images of cofilin staining in cells transfected with RNAi as indicated. f Quantification and g representative images of the TNT formation in non-treated HBEC-3 cells incubated with supernatant obtained from siNEG or siRASSF1A treated cells during 24 h. The TNTs are show with arrowheads. Values are the mean ± SEM of three independent experiments in approximately 200 cells. Statistical significance was calculated and p value are indicated by asterisks: **p < 0.01

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