Fig. 3
From: A role for RASSF1A in tunneling nanotube formation between cells through GEFH1/Rab11 pathway control
TNTs induced by RASSF1A depletion still occurs in either hypoxic or serum starved conditions. (a) Representative images of HIF-1α in HBEC-3 cells incubated for 24 h in normoxic or hypoxic (0.1% O2) condition after transfection with either control or RASSF1A RNAi. (b) Representative images of the increased number of multi-nucleus HBEC-3 cells, as the result of serum deprivation compared to the cells incubated in normal condition with intact nuclei. (c) Quantification of TNT number in control and RASSF1A depleted HBEC-3 cells after incubation in either hypoxic or serum starved conditions and (d) Representative images (i and ii are zoom of the corresponding arrowheads for respectively control condition, hypoxia or deprivation treatment). The TNTs are show with arrowheads. Roman numerals mark the examples of the TNT in the zoomed images. Values are the mean ± SEM of three independent experiments in approximately 200 cells. Statistical significance was calculated and p value are indicated by asterisks: *p < 0.05, **p < 0,01; *** < 0,001