Fig. 5
From: Pharmacological inhibition of LSD1 activity blocks REST-dependent medulloblastoma cell migration
Pharmacological inhibition of LSD1 alters expression of MB and migration related genes. a, Volcano plot of expression changes from RNA-sequencing data. 451 altered genes with p < 0.05. b, Ingenuity pathway analysis of function revealed top functions outside of cancer. c, Heat map of log2fold gene expression changes in the cell adhesion and migration function. Orange arrow highlights HIF1A. d, qPCR analysis of HIF1A expression in Daoy and UW228 cells treated with GSKLSD1 (**p < 0.01; ****p < 0.0001). e, qPCR analysis of HIF1A expression in Daoy-REST and UW228-REST cells treated with GSKLSD1 (p = 0.07; ****p < 0.0001). f, Scratch wound migration assay of UW228, UW228-REST, Daoy, and Daoy-REST cells in three conditions: untreated (UT), GSKLSD1 (75 uM), or GSKLSD1-HIF1A (75 uM) (*p < 0.05, **p < 0.01, ***p < 0.001). g, Bar plot of HIF1A expression in Clusters 2, 3, 4, and 5 (***p < 0.001; ****p < 0.0001). h, Scatter plot of correlation of REST and HIF1A across Clusters 2, 3, 4, and 5 (r = 0.36, p < 0.001). I, Scatter plot of correlation of LSD1 and HIF1A across Clusters 2, 3, 4, and 5 (r = 0.27, p < 0.01)