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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Pharmacological inhibition of LSD1 activity blocks REST-dependent medulloblastoma cell migration

Fig. 3

LSD1 expression is increased by elevated REST and cell growth is blocked by LSD1 knockdown in SHH MB cell lines. a, Western blot of REST and LSD1 protein levels in cell pairs (UW228, Daoy) with and without the human REST transgene (hREST; UW228-REST, Daoy-REST) (left panel). Quantification of REST (black) and LSD1 (grey) protein density normalized to actin loading control (right panel; **p < 0.01, ****p < 0.000). b, qPCR analysis of REST (black) and LSD1 (grey) expression normalized to 18S (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). c, qPCR analysis of LSD1 expression in UW228, Daoy, and Daoy-REST cells treated with shLSD1 (*p < 0.05, **p < 0.01, ****p < 0.0001). d, Western blot of REST, LSD1, Caspase 3, Cleaved Caspase 3, and actin protein levels in control (ctrl) and shLSD1 treated cells. e, Cell viability as measured by trypan blue counting in UW228, Daoy, and f, Daoy-REST cells. Graphs represented as viable cell count/mL and % cell viability. Pictures represent cell confluency at 24, 48, and 72 h post-transduction (*p < 0.05; **p < 0.01;***p < 0.001)

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