Fig. 3
From: IGFBP6 controls the expansion of chemoresistant glioblastoma through paracrine IGF2/IGF-1R signaling
IGFBP6 and IGF2 are differentially secreted by TMZ-sensitive and TMZ-resistant glioma cells. a Determination of IGF2 and IGFBP6 expression in conditioned medium of TMZ-sensitive (U251, left) and TMZ-resistant (UTMZ, right) glioma cells using a human growth factor antibody array. b Quantitative analysis of IGF2 (left) and IGFBP6 (right) levels in culture medium of U251 and UTMZ glioma cells determined by ELISA. c Relative expression of IGF2 (left) and IGFBP6 (right) mRNA in tumor samples from GBM patients with OS less than or greater than 6 months. d Relative expression of IGF2 (left) and IGFBP6 (right) mRNA in TMZ-sensitive and TMZ-resistant GBM xenograft lines. mRNA expression was determined by qRT-PCR and normalized to 18S. *P < 0.05, **P < 0.001, ****P < 0.0001. Two-tailed, t-test