Fig. 7
From: TEM8 functions as a receptor for uPA and mediates uPA-stimulated EGFR phosphorylation
Effect of uPA/TEM8 interaction on tumor growth and metastasis. a For the migration assay, HepG2 cells (1 × 105 cells/well) were seeded into the upper chambers of Transwell plates coated with 3 μg/ml collagen. Cells were stimulated with or without LMW-uPA in the presence or absence of TEM8-Fc for 4 h. Migrated cells were quantified as described in Materials and Methods. Similar results were obtained in three independent experiments; “a” indicates p < 0.001 vs. control group; “b” indicates p < 0.001 vs. LMW-uPA group. b The expression of uPA, TEM8, EGFR and uPAR and the ERK1/2 phosphorylation were examined on serial frozen cancer tissue sections by immunohistochemistry or immunofluorescence. c TEM8-Fc and ATF-Fc, at the indicated doses, substantially blocked the growth of subcutaneously implanted MCF-7 breast tumor cells in nude mice, when administered every 2 days for 3 weeks. * p < 0.01, ** p < 0.001 vs. control group. AF + TF refers to combined ATF-Fc and TEM8-Fc