Fig. 6

miR-32-5p suppressed BIK expression by targeting KLF4. a-b miR-32-5p was transfected into PC3 and DU145 cells. Cell lysates were analysed using the indicated antibodies. The mRNA levels of BIK were detected by q-RT-PCR. Data represent the mean ± SD of three independent experiments. **p < 0.01 vs. control. c-f KLF4 was transfected into PC3 and DU145 cells with or without miR-32-5p overexpression. Cell lysates were analysed using the indicated antibodies. The mRNA levels of BIK were detected by q-RT-PCR. Data represent the mean ± SD of three independent experiments. ***p < 0.001 vs. control. g-h miR-32-5p was transfected into PC3 cells and then the cells were treated with 20 μM cisplatin at the indicated times. Cell lysates were analysed using the indicated antibodies. The mRNA levels of BIK were detected by q-RT-PCR. Data represent the mean ± SD of three independent experiments. ***p < 0.001 vs. control. i Flag-KLF4 was transfected into PC3 cells with or without miR-32-5p overexpression and then the cells were treated with 20 μM cisplatin at the indicated times. Cell lysates were analysed using the indicated antibodies. The mRNA levels of BIK were detected by q-RT-PCR. Data represent the mean ± SD of three independent experiments. ***p < 0.001 vs. control