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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Mast cells and mast cell tryptase enhance migration of human lung fibroblasts through protease-activated receptor 2

Fig. 6

Effect of PAR2 antagonist on migratory capacity of HFL-1. The PAR2 antagonist P2pal-18S (10 μM), did not have any significant effect on the migratory capacity of the HFL-1 (a). P2pal-18S inhibited the pro-migratory effect of tryptase at 48 h and 72 h (b), and in co-cultures of fibroblasts and LAD2 cells at 48 h and 72 h (c). Conditioned medium (CM) increased the fibroblast migration, in presence (48 h and 72 h) and absence (72 h) of stimulation with P2pal-18S (d). The enhanced migration of HFL-1 caused by LAD2 decreased from 28 to 4% at 48 h, after stimulation with PAR2 antagonist, P2pal-18S. Tryptase enhanced the HFL-1 migration by 21%, while this effect was reduced to 0.015% at 48 h, after stimulation with P2pal-18S (e). The migratory capacity of HFL-1 cells was measured at 24, 48 and 72 h as the percentage of cell-occupied space compared to time point 0 h. The statistical analysis was performed using linear mixed models (mean ± SD, n = 3–4 individual experiments with 2–3 technical replicates in each experiment, *p < 0.05, **p < 0.01 and ***p < 0.001)

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