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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Mast cells and mast cell tryptase enhance migration of human lung fibroblasts through protease-activated receptor 2

Fig. 3

Effect of mast cell degranulation on HFL-1 migration. Conditioned medium from LAD2 cells enhanced the migration capacity of HFL-1 (a). However, there was no difference in migration between HFL-1 cells that were treated with conditioned medium from IgE/Anti IgE stimulated LAD2 cells, compared to non-stimulated cells (a). Representative images of HFL-1 cells from starting point at 0 h (upper panels) and after 72 h (lower panels), HFL-1 controls and fibroblasts stimulated with conditioned medium from LAD2 cells (b). The migratory capacity of HFL-1 cells was measured at 24, 48, 72 h as the percentage of cell-occupied space compared to time = 0 h when the scratch was made (mean ± SD, n = 3 individual experiments with 2 technical replicates in each experiment). Conditioned medium did not have any proliferative effects on HFL-1 neither in 0.4% nor 10% serum concentration (c). Proliferation of HFL-1 was measured at 24, 48 and 72 h after stimulation (mean ± SD, n = 3 individual experiments with 4–6 technical replicates in each experiment, *p < 0.05, **p < 0.01 and ***p < 0.001)

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Cell Communication and Signaling

ISSN: 1478-811X