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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Activation of the JAK/STAT3 and PI3K/AKT pathways are crucial for IL-6 trans-signaling-mediated pro-inflammatory response in human vascular endothelial cells

Fig. 4

The effect of different inhibitions on IL-6 trans-signaling induced MCP-1 mRNA expression. The effect of a CP960550 (JAK inhibitor, 10 μM), b STAT3 knockdown, c LY294002 (PI3K inhibitor, 50 μM) and d PD98059 (MEK inhibitor, 10 μM) on trans-signaling (IL-6 = 100 ng/ml, sIL-6R = 200 ng/ml) induced MCP-1 expression is presented as mean ± SEM (n = 3 for each). Western blots show effect of CP960550, STAT3 knockdown, LY294002 and PD98059 on IL-6 trans-signaling (IL-6 = 50 ng/ml, sIL-6R = 100 ng/ml) induced phosphorylation of downstream pathway proteins. *p < 0.05, **p < 0.01, ***p < 0.001 compared to control

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