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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Activation of the JAK/STAT3 and PI3K/AKT pathways are crucial for IL-6 trans-signaling-mediated pro-inflammatory response in human vascular endothelial cells

Fig. 1

Expression and regulation of IL-6R and gp130 in human vascular endothelial cells. Flow cytometry analyses of EDTA detached endothelial cells stained for a IL-6R and b gp130. The light grey histograms show respective FMO (fluorescence minus one) controls and the dark grey histograms show IL-6R or gp130 stained cells. ELISA analyses of the levels of c IL-6R and d gp130 in cell lysates and medium after 48 h of IL-6 knockdown. ELISA analyses of the levels of e IL-6R and f gp130 in cell lysates and medium after 48 h of treatment with TNF-α (50 ng/ml) and LPS (100 ng/ml). Data is presented as mean ± SEM of at least 3–4 experiments each run-in duplicate. *p < 0.05, **p < 0.01, ***p < 0.001 compared to control

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