Fig. 5

RHBDD1 deletion decreases the protein levels of p-Akt and CDK2. a The protein levels of Akt, p-Akt, CDK2, cyclinE1, p27, CDK4 and cyclinD1 were analyzed by western blot using GAPDH as a loading control. Experiments were repeated three times. b Western blot analysis for Akt, p-Akt, CDK2, cyclinE1, p27, CDK4 and cyclinD1 expression in MCF7 wild-type and knock-out cells at the indicated time points after double thymidine treatment. GAPDH was a loading control. Experiments were repeated three times. c Western blot analysis for Akt, p-Akt, CDK2, cyclinE1, p27, CDK4 and cyclinD1 expression in MDA-MB-231 wild-type and knock-out cells at indicated time points after double thymidine treatment. GAPDH was a loading control. Experiments were repeated three times. d CDK2 protein levels of wild-type and knock-out MCF7 and MDA-MB-231 cells were analyzed by western blot assay. Cells were treated with 10 μM CHX, along with MG132 (1 μM for MCF7 and 2 μM for MDA-MB-231) or vehicle DMSO control, and samples were collected at the indicated times. Both RHBDD1 and GAPDH were loading controls. Experiments were repeated three times