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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Rhomboid domain-containing protein 1 promotes breast cancer progression by regulating the p-Akt and CDK2 levels

Fig. 4

RHBDD1 deletion suppresses cell cycle progression and blocks G1/S transition. a DNA content of wild-type and knock-out MCF7 cells were determined at indicated time points after double thymidine treatment (means±s.d., two-way ANOVA, * p < 0.05). Experiments were repeated five times. b DNA content of wild-type and knock-out MDA-MB-231 cells were determined at indicated time points after double thymidine treatment (means±s.d., two-way ANOVA, * p < 0.05). Experiments were repeated five times. c Heatmap of cell cycle related genes differentially expressed in MCF7 wild-type and knock-out cells using R software. The log2 values were calculated for each sample by normalizing to the number of reads alone (p < 0.05, FDR < 0.05). d Gene Set Enrichment Analysis (GSEA) of genes involved in cell cycle progression in MCF7 RHBDD1 knock-out cells with the gene sets corresponding to CELL CYCLE in the KEGG database (NES Score = −1.40, FDR = 0.013). e GSEA of genes involved in G1/S phase transition in MCF7 RHBDD1-knockout cells with the gene sets corresponding to GO_CELL_CYCLE_G1_S_PHASE_TRANSITION in the MSigDB database (NES Score = − 1.49, FDR = 0.0025). f Relative mRNA expression of GATA6, SOX9, JUNB, BRINP1 and NUPR1 in MCF7 wild-type and knock-out cells by quantitative real-time PCR, normalized to GAPDH (means±s.d., t test, * p < 0.05). Experiments were repeated three times

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