Fig. 1

Induction of PRL3 expression by anticancer drugs in cancer cells. a PRL3 expression is upregulated in B16F0 cells upon treatment with various anticancer drugs. B16F0 cells were treated with 5 μM etoposide, 50 μM cisplatin, 2.5 μM doxorubicin, or 2 μM paclitaxel for 24 h. b, d Dose dependency of PRL3 induction upon doxorubicin and cisplatin administration. B16F0 cells were treated with the indicated amounts of drugs for 24 h. c, e Time dependency of PRL3 induction upon doxorubicin or cisplatin administration. B16F0 cells were treated with 2.5 μM doxorubicin or 50 μM cisplatin. PRL3 expression was analyzed 24 h after treatment or after a second 24 h incubation period without treatment. f PRL3 upregulation upon doxorubicin treatment is a characteristic feature of B16F0, B16F1, and B16F10 cells. The three B16 cell lines were treated with 2.5 μM doxorubicin for 24 h