Fig. 6

TEM derived VEGF stimulated cancer cell EMT through Nrf2 activation. a the migrated cancer cell stimulated with CM of TEM with (TEM-CM + anti-VEGF) or without (TEM-CM + IgG) VEGF neutralization were observed and counted (scale bar 100 μm) (n = 3). b the cancer cell morphology changes were observed after stimulated with CM of TEM with (TEM-CM + anti-VEGF) or without (TEM-CM + IgG) VEGF neutralization (scale bar 50 μm). c E-cadherin and N-cadherin expression of cancer cell stimulated with CM of TEM with (TEM-CM + anti-VEGF) or without (TEM-CM + IgG) VEGF neutralization were detected by western blotting. d nuclear Nrf2 of cancer cell was measured after stimulated with CM of TEM with (TEM-CM + anti-VEGF) or without (TEM-CM + IgG) VEGF neutralization were detected by western blotting. e HO-1 and Nqo-1 expression of cancer cells were detected by qPCR after stimulated with CM of TEM or VEGF neutralized (anti-VEGF) CM of TEM (n = 4). f The working model of how the cancer cells and macrophages interaction. Graphs show the data as mean ± SD. *, P < 0.05