Fig. 5

TEM activated Nrf2 of cancer cells was correlated with cancer cell EMT. a the tumor tissue of mice xenograft model stained with CD163 (green), Nrf2 (red) and DAPI (blue) (scale bar 100 μm) (n = 4). b nuclear Nrf2 of cancer cell was measured after stimulated with FBS-free medium (Ctrl), CM of M0 macrophages (M0-CM) or TEM (TEM-CM) by western blotting. c nuclear Nrf2 level of cancer cells after Nrf2 knocked down (si-Nrf2) and stimulated with TEM-CM or M0 CM were detected by western blotting. d the HO-1 and Nqo-1 expression of cancer cells were detected by PCR (n = 4). e the migrated cells of normal stimulated with M0 CM (M0-CM) and TEM CM (TEM-CM) and Nrf2 knocked down cancer cell stimulated with TEM CM (TEM-CM + si-Nrf2) were observed and counted (scale bar 100 μm) (n = 3). f the cancer cell morphology changes were observed after 24 h incubation with the M0 CM (M0-CM), TEM CM (TEM-CM) or Nrf2 knockdown followed TEM CM (TEM-CM + siNrf2) (scale bar 50 μm). g E-cadherin and N-cadherin expression of normal and Nrf2 knocked down cancer cell were detected by western blot, after 24 h incubation with the M0 CM (M0-CM), TEM CM (TEM-CM) or Nrf2 knockdown followed TEM CM (TEM-CM + siNrf2). Graphs show the data as mean ± SD. *, P < 0.05, compared between two groups