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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Nrf2 activation drive macrophages polarization and cancer cell epithelial-mesenchymal transition during interaction

Fig. 2

Nrf2 activation was correlated with M2 TEM formation and VEGF-α expression. a the siRNA of Nrf2 and Keap-1 and DEM were used to pre-modulate Nrf2 activation. The nuclear Nrf2 were detected by western blotting. b the M1/2 macrophages markers of Nrf2 knocked-down (si-Nrf2) were detected after cancer cell CM stimulation (n = 4). c 24-h secretion of IL-1, IL-6 and VEGF of Nrf2 knocked-down TEM were evaluated by ELISA (n = 3). d the M1/2 macrophages markers of Keap-1 knocked-down (si-Keap-1) were detected after cancer cell CM stimulation (n = 4). e IL-1, IL-6 and VEGF concentration of Keap-1 knocked-down (si-Keap-1) TEM were evaluated by ELISA (n = 3). f M0 macrophages were pre-treated with 100-mM DEM for 6 h before cancer cell CM stimulation, the M1/2 macrophages markers were detected by PCR (n = 4). g, IL-1, IL-6 and VEGF concentration of DEM pre-treated (DEM) were evaluated by ELISA (n = 3). Graphs show the data as mean ± SD. *, P < 0.05, compared with si-Neg or ctrl

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