Fig. 8

Co-cultivation of murine bone marrow stromal cells with MCF-7 breast cancer cells induces increases in secretion of IL-6 and IL-8. Confluent stroma were treated with H₂O₂ 100 μM for an hour, washed once with PBS, medium was changed to DMEM/5%FCS and conditioned medium was sampled for injury-induced IL-6 and IL-8 production as positive controls. Simultaneously, medium on uninjured stroma in triplicate wells were changed to DMEM/5%FCS containing variable numbers of MCF-7 cells and incubated for 48 h. Conditioned medium samples were collected 48 h after incubation and (a). IL-6 and (b). IL-8 concentrations were determined using murine IL-6 and CXCL1/KC ELISA kits, respectively, according to manufacturer’s instructions. Error bars: Standard Deviations. *p < 0.05, **p < 0.01 ***p < 0.001