Skip to main content
Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Wilms’ tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90

Fig. 2

WTAP supports proliferation and counteracts apoptosis of DLBCL cells. a OCI-Ly19 and OCI-Ly10 cells, 48 h post-infection. Expression of WTAP was checked on the level of protein and mRNA by using western blotting and qPCR. Mean ± s.d. of three technical replicates were plotted. **P < 0.01, ***P < 0.001. b, c We infected OCI-Ly19 and OCI-Ly10 by WTAP-overexpressing lentivirus or WTAP-knocked down lentivirus. Then cells were cultured in 96 cell panels for 0, 24, 48, and 72 h. Cell viability was measured by CCK8 assay.*, P < 0.05, **, P < 0.01 and ***, P < 0.001 compared with control group. d OCI-Ly19-WTAP and the control group, OCI-Ly19-control, were cultured in serum deprivation medium and treated with hydrogen peroxide (300 mM) for 16 h. The apoptosis assay was detected by flow cytometry. *P < 0.05 compared with the control group. e OCI-Ly10-shWTAP and OCI-Ly10-control cells were cultured in medium with Etoposide (10 μM) or DMSO for 24 h. f The apoptosis was assayed by flow cytometry. *, P < 0.05 compared with the control. The quantitative analysis of the percentage of positive cells was reported from three independent experiments

Back to article page