Fig. 1
Kv1.3 channel blockade does not compromise cell viability. Purified CD4+ TEM cells were stained for the (a) CD3, CD4 and (b) CD45RO and CCR7 surface markers. (c) Cell viability was assessed following a 24 h culture period with the Fixable Viability Dye eFluor 780. Changes in FSC and positive staining with the viability dye were considered as cell death markers. For death positive control, 30% dimethyl sulfoxide (DMSO) was added to the cells for 30 min. Data from three independent experiments are shown as mean ± SEM (standard error of mean). (d) Kv1.3 channels currents were measured by patch clamp in whole cell mode. Currents were evoked by a depolarizing pulse to + 50 mV from a − 120 mV holding potential. The Vm24 toxin was perfused to the cells at a concentration of 1 nM