Fig. 1

The Ras subfamily and their regulators, overview of analyses performed, and Spearman’s rank correlations. a Schematic diagram of the Ras superfamily of small GTPases that cycle between a GDP-bound “OFF-state” and a GTP-bound “ON-state”. This cycling is regulated by GEF and GAP proteins. b Number of Ras subfamily and regulators genes in four species (Homo sapiens, hs; Mus musculus, mm; dr, Danio rerio, dr; Drosophila melanogaster, dm). c Schematic figure of protein and gene expression datasets used, and main data analyses performed in this study. d Spearman’s rank correlation values of the ∑RAS vs ∑GEF, ∑RAS vs ∑GAP, and the ∑GEF vs ∑GAP displayed as a heat map (normal tissue datasets dmNT_01, ggNT_01, oaNT, mdNT_01, mmNT_01, mmNT_02, mmuNT_01, ppyNT_01, ptNT_01, ppNT_01, ggoNT_01, hsNT_01, hsNT_02, hsNT_03, hsNT_04, hsNT_05). e Heatmap representation of Spearman’s rank correlations of protein levels for GEFs with RASs, GAPs with RASs, and GEFs with GAPs across human normal tissues. While correlations between the GEF with the RAS and the GAP with the RAS were generally poorer, higher correlations between the GEF with the GAP were observed independently in both proteomic datasets. f Heatmap representation of Spearman’s rank correlations of protein and RNA levels (hsNT_03). Parts of the pictures were drawn using Inkscape