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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton

Fig. 1

UT-SCC-42B and UT-SCC-19B carcinoma cells were embedded in 3D collagen after shPPFIA1_69 or shScr (control) transduction and cultured for seven days. a Representative phase contrast light micrographs showed less cellular outgrowths and invasive phenotype in colonies transduced with shPPFIA1_69 compared to shScr control cells. b Quantification of cell invasive growth measured by relative area of the colonies; mean ± SEM; three collagen preparations/stable control (shScr) or knockdown (shPPFIA1_69) cell line. *P < 0.001, unpaired Student’s t-test. c Western blot confirmed the efficacy of liprin-α1 knockdown in UT-SCC-19B and UT-SCC-42B cell lines. d Quantification of relative colony and lumen area of UT-SCC-42B cells in 3D collagen with shScr and shPPFIA1_97; mean ± SEM; three collagen preparations/stable control (shScr) or knockdown (shPPFIA1_97) cell line. *P < 0.01, unpaired Student’s t-test. Scale bar 10 μm. e Lumen formation was diminished in liprin-α1 knockdown cell colonies compared to shScr cells as visualized by Z-stacks. f Representative confocal micrographs illustrated liprin-α1 (green) localization in the cytosol and at the cell-extracellular matrix contacts including focal adhesion-like cellular outgrowths. Phalloidin staining (filamentous actin, red) visualizes reduced growth and changes in cytoskeletal elements after liprin-α1 knockdown. g Confocal micrographs showed vinculin localization at the cell colonies. Scale bar 50 μm

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