Fig. 6

Leptomycin B, both singly and in combination with chemotherapeutic agents, modulates the expression of crucial apoptotic pathway genes in a cell-type specific manner a A2780CP and b ECC-1 cell lines were treated with leptomycin B (20 nM), cisplatin (10 μM), TRAIL (100 ng/mL) or a combination of leptomycin B with cisplatin or TRAIL for 24 h. They were then subjected to RT-qPCR analysis to quantify the mRNA expression of DR4, DR5, p21, p27, DcR1, DcR2 and PUMA. 18S mRNA expression was used as control for qPCR results. Results shown are representative of three independent experiments. Brackets are used to show statistical differences between treatment groups. All data are means ± SEM of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001