Fig. 2

Combination of cisplatin or TRAIL with leptomycin B synergistically induces apoptosis induction in endometrial cancer cell lines. a Endometrial cancer cell lines were treated with leptomycin B (20 nM), cisplatin (10 μM), TRAIL (100 ng/mL) or a combination of leptomycin B with cisplatin or TRAIL for 24 h. Western blot was performed using relevant antibodies and β-Actin was used as a loading control. Results shown are representative of three independent experiments. b Densitometric analysis of PARP cleavage followed by 2-way ANOVA analysis; bracket indicate when interaction was statistically significant. c Flow cytometry analysis was performed on the cells by staining with annexin V/PI and the levels of cell death was measured; cells stained with annexin V and/or PI were used to determine the relative quantification of cell death. 2-way ANOVA was performed on the data; brackets indicate when interaction was statistically significant. All data are means ± SEM of three independent experiments. *, p < 0.05; **, p < 0.01