Fig. 3
From: Interleukin-15 facilitates muscle regeneration through modulation of fibro/adipogenic progenitors
IL-15 stimulates proliferation of FAPs both in vivo and in vitro. a A schematic showing the experiment in vivo: IL-15 (or combination with Jak inhibitor) was administered from 3dpi to 5 dpi and samples were sectioned on 7 dpi (top). Activated FAPs were detected by staining Ki67 (bottom). Scale bars, 20 μm. b Quantifications of percentage of PDGFRα+Ki67+ FAPs in total FAPs. c The ability of proliferation of FAPs in the presence of IL-15 (or combination with Jak inhibitor) detected by BrdU staining. d Quantifications of percentage of BrdU+ FAPs in total FAPs. e Western blots for activation of Jak-STAT pathways after stimulated by IL-15 with/without inhibitor and (f) quantity analysis. g FITC-Annexin-V/PI assay for apoptosis of FAPs after stimulated by IL-15 for 48 h. Values not sharing a common small letter differ significantly (p < 0.05). Abbreviations: Gly, glycerol; I, Inhibitor