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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Fascin induces melanoma tumorigenesis and stemness through regulating the Hippo pathway

Fig. 6

Fascin interacts with MST2 and reduces MST2 homodimeration and p-LATS. a WM793 fascin KO cells were reintroduced with fascin-WT and fascin-S39E plasmids. Western blot analysis was performed using indicated antibodies. b HEK293T cells were transfected with the indicated plasmids. Following incubation for 48 h, cell lysates were immunoprecipitated with Flag (M2) antibody and then immunoblotted with GFP antibody. c In vitro translated [35S]-MST2-full length (FL), MST2(1-313aa) and MST2(314-491aa) truncated proteins were incubated with GST and GST-fascin fusion proteins. After wash, protein complex was eluted and then separated on 10% SDS-PAGE and exposed to phospho-imager (Amersham) for autoradiography. d HEK293T cells were transfected with indicated plasmids. Cell lysates were immunoprecipitated with Flag (M2) antibody and then immunoblotted with GFP antibody. e A proposed model of TAZ regulation by Fascin. Fascin binds to MST2 and inhibits MST2 homodimer formation and kinase activity, which results in inhibition of LATS1/2 activation and TAZ phosphorylation, and thus inhibits TAZ degradation

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