Fig. 5

Verification of the role of PTEN-Δ. a The PTEN-Δ expression value of Caki-1 cells (native highly PTEN-Δ expressing) after PTEN-Δ siRNA knockdown (KD) was determined by Real-Time PCR. Expression was normalized to expression of the housekeeping genes TBP, ATP5J and PPIA and is shown as percentage of the expression of the nonsense siRNA transfection control (scrambled locus). Expression of PTEN-Δ was reduced to 32%. b Migration of Caki-1 cells after PTEN-Δ siRNA knockdown was determined in a Boyden chamber using fibronectin (10 μM) as chemotaxin. The migration value is shown as percentage of the migration of the scrambled locus siRNA transfection control. c Integrin α5 expression value of Caki-1 cells after PTEN-Δ knockdown was determined by flow cytometry. The expression value is shown as percentage of the transfection control (scrambled locus siRNA transfected negative control). Phosphorylation of AKT (S473) (d) and p38 (T180/Y182) (e) in Caki-1 cells after PTEN-Δ knockdown was determined by Western blot and quantified by densitometric evaluation of the bands. Results are shown as percentage of siRNA control transfected cells (scrambled locus). Significance was calculated by Student’s T-test, *p < 0.05